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Epithelial–mesenchymal interactions induce enamel matrix proteins and proteases in the epithelial cells of the rests of M alassez in vitro
Author(s) -
Takahashi Ken,
Shimonishi Mitsuru,
Wang Rui,
Watanabe Hiroatsu,
Kikuchi Masahiko
Publication year - 2012
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.2012.01002.x
Subject(s) - proteases , in vitro , mesenchymal stem cell , viral matrix protein , microbiology and biotechnology , chemistry , matrix (chemical analysis) , enamel paint , extracellular matrix , biology , biochemistry , enzyme , gene , medicine , dentistry , chromatography
Epithelial–mesenchymal interactions influence morphogenesis and cell differentiation in periodontal tissue regeneration. The current study examined the expression of amelogenin, ameloblastin, matrix metallopeptidase‐20 ( MMP ‐20), and kallikrein‐4 ( KLK ‐4) and their effects on the interactions between the epithelial cells of Malassez and periodontal ligament fibroblasts. Explants of human periodontal ligament tissues produced outgrowths containing both the epithelial cells of Malassez and periodontal ligament fibroblasts after incubation in a modified serum‐free medium. Both the epithelial cells and fibroblasts were co‐cultured in the same dish. The distribution and expression of all four factors were evaluated using immunohistochemistry, in‐situ hybridization and RT ‐ PCR analysis. The epithelial cells of Malassez were cultured separately and were used as the control. Immunohistochemical analysis revealed weak expression of amelogenin, ameloblastin, MMP ‐20 and KLK ‐4 in epithelial cells of Malassez co‐cultured with periodontal ligament fibroblasts. in‐situ hybridization and RT ‐ PCR confirmed significant mRNA expression of these factors in co‐cultured cells compared with control cells. MMP 20 mRNA was not expressed in control cells. These results suggest that the epithelial–mesenchymal interactions promote differentiation of human epithelial cells of Malassez and that the induction of enamel matrix proteases facilitates the degradation of enamel matrix proteins.