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Synaptic vesicle protein 2b is expressed temporospatially in (pre)odontoblasts in developing molars
Author(s) -
Yang SoYoung,
Jeon SooKyung,
Kang JeeHae,
Yoo HongIl,
Kim YooSeong,
Moon JungSun,
Kim MinSeok,
Koh JungTae,
Oh WonMann,
Kim SunHun
Publication year - 2012
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.2012.01001.x
Subject(s) - odontoblast , ameloblast , microbiology and biotechnology , chemistry , gene isoform , dentinogenesis , secretion , vesicle , dentin , biology , biochemistry , enamel paint , pathology , gene , medicine , dentistry , membrane
The formation of dentin and enamel is initiated by the differentiation of odontogenic precursor cells into odontoblasts and ameloblasts, respectively. This study was performed to identify new molecules involved in the differentiation of odontogenic cells. The genes expressed differentially between the root stage (after the differentiation of odontogenic cells and dental hard‐tissue formation) and the cap stage (before the differentiation of odontogenic cells and dental hard‐tissue formation) were searched using differential display PCR . For the first time, synaptic vesicle protein ( SV ) 2b, an important transmembrane transporter of C a 2+ ‐stimulated vesicle exocytosis, was identified as a differentially expressed molecule. Real‐time PCR and western blotting revealed an increase in the transcriptional and translational levels of SV 2b during or after the differentiation of odontogenic cells. Immunofluorescence revealed this molecule to be localized in not only fully differentiated odontoblasts but also in pre‐odontoblasts before dentin matrix secretion. The expression pattern of the SV 2a isoform was similar to that of the SV 2b isoform, whereas the SV 2c isoform showed a contrasting pattern of expression. After treatment with alendronate, an inhibitor of protein isoprenylation for the transport of secretory vesicles, the expression of SV 2a and SV 2b decreased, whereas that of SV 2c increased. These results suggest that the SV 2 isoforms are functional molecules of (pre)odontoblasts which may be involved in vesicle transport.