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Immunomodulation of dendritic cells differentiated in the presence of nicotine with lipopolysaccharide from P orphyromonas gingivalis
Author(s) -
Yanagita Manabu,
Mori Kenta,
Kobayashi Ryohei,
Kojima Yuko,
Kubota Mikiko,
Miki Koji,
Yamada Satoru,
Kitamura Masahiro,
Murakami Shinya
Publication year - 2012
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.2012.00992.x
Subject(s) - chemokine , lipopolysaccharide , t cell , immunology , dendritic cell , chemistry , immune system , proinflammatory cytokine , nicotine , monocyte , microbiology and biotechnology , innate immune system , porphyromonas gingivalis , acquired immune system , biology , inflammation , periodontitis , medicine , neuroscience
Tobacco smoking is a significant risk factor for periodontal diseases. Nicotine, one of the most studied constituents in cigarette smoke, is thought to modify immune responses. Dendritic cells ( DC s), which are key mediators between innate and adaptive immunity, stimulate naive T cells to differentiate to effector T ‐cell subsets that may be actively involved in the immunopathogenesis of periodontal diseases. In this study, we evaluated the effects of nicotine and lipopolysaccharide ( LPS ) from P orphyromonas gingivalis , alone and in combination, on the functions of human monocyte‐derived DC s to elucidate the mechanism of tissue destruction of smoking‐associated periodontal diseases. P . gingivalis LPS ‐stimulated DC s differentiated with nicotine ( N i DC s) induced lower T ‐cell proliferation and human leukocyte antigen ( HLA )‐ DR expression, but elevated expression of programmed cell death ligand 1. Additionally, N i DC s impaired interferon‐ γ production but maintained interleukin ( IL )‐5 and IL ‐10 production in co‐cultured T cells. Furthermore, N i DC s produced lower levels of proinflammatory cytokines compared with DC s differentiated in the absence of nicotine. Interestingly, N i DC s preferentially produced the T helper 2 ( T h2)‐type chemokines macrophage chemotactic protein‐1 and macrophage‐derived chemokine. These results suggest that the presence of nicotine during differentiation of DC s modulates the immunoregulatory functions of P. gingivalis LPS ‐stimulated DC s.