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Molecular structural analysis of carious lesions using micro‐ R aman spectroscopy
Author(s) -
Levallois Bernard,
Terrer Elodie,
Panayotov Yvan,
Salehi Hamideh,
Tassery Hervé,
Tramini Paul,
Cuisinier Frédéric
Publication year - 2012
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.2012.00988.x
Subject(s) - raman spectroscopy , chemistry , fluorescence , maillard reaction , nitric acid , aqueous solution , fluorescence spectroscopy , remineralisation , lactic acid , dentistry , biochemistry , bacteria , organic chemistry , inorganic chemistry , fluoride , medicine , physics , genetics , biology , optics , quantum mechanics
In clinical situations carious dentine tissues can be discriminated by most caries fluorescence detection tools, including a new fluorescence intra‐oral camera. The objectives of this study were: (i) to analyze the R aman spectra of sound, carious, and demineralized dentine, (ii) to compare this spectral analysis with the fluorescence variation observed when using a fluorescence camera, and (iii) to evaluate the involvement of the M aillard reaction in the fluorescence variations. The first positive hypothesis tested was that the fluorescence of carious dentine obtained using a fluorescence camera and the R aman spectra variation were closely related. The second was that the variation of fluorescence could be linked with the M aillard reaction. Sound dentine, sound dentine demineralized in aqueous nitric acid solution, carious soft dentine, sound dentine demineralized in lactic acid solution, sound dentine demineralized in aqueous nitric acid solution and immersed in methylglycoxal solution, and sound dentine demineralized in aqueous nitric acid solution and immersed in methylglycoxal and glucose solutions, were studied using micro‐ R aman spectroscopy. Modifications in the band ratio of amide, phosphate, and carbonate were observed in the decayed and demineralized groups compared with the sound dentine group. The results indicate that a close relationship exists between the M aillard reaction and fluorescence variation.