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Initial responses of osteoblasts derived from human alveolar bone to various compressive forces
Author(s) -
Tripuwabhrut Polbhat,
Mustafa Kamal,
Brudvik Pongsri,
Mustafa Manal
Publication year - 2012
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.2012.00980.x
Subject(s) - chemistry , viability assay , apoptosis , osteoblast , microbiology and biotechnology , mtt assay , interleukin 8 , chemokine , cell growth , messenger rna , bone remodeling , cytokine , immunology , in vitro , medicine , receptor , biology , biochemistry , gene
Mechanical stress generated by orthodontic force is recognized as a major factor in the modulation of alveolar bone remodeling. During this process, osteoblasts play a crucial role, not only by participating in bone formation but also by promoting osteoclastogenesis. The aim of this study was to investigate how continuous compressive force ( CF ) affects human primary osteoblasts ( HOB s) in terms of cell proliferation, apoptosis, and expression of interleukin‐6 ( IL ‐6) and chemokine CXC ligand 8 ( CXCL 8). Human primary osteoblasts, isolated from human mandibular bone pieces, were cultured with or without CF (1–4 g cm −2 ) for up to 72 h. Cell viability and proliferation were evaluated using the MTT assay. RT‐PCR was used to determine the levels of expression of KI67 (a proliferation marker), BAX (a pro‐apoptotic marker), BCL 2 (an apoptotic inhibitor), IL 6 , and CXCL 8 mRNA s, while a multiplexed bead immunoassay was used to measure the release of IL‐6 and CXCL8. The results revealed that CF decreased cell viability and proliferation in a time‐ and force‐dependent manner. After applying CF for 24 h, the mRNA expression of KI67 was markedly inhibited, whereas the mRNA expression of BAX and BCL2 was unaltered. In addition, CF enhanced the levels of IL 6 and CXCL 8 mRNA s in a force‐dependent manner, whereas the levels of the corresponding proteins were reduced in the compressed HOB s.