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Detection and quantification of monomers in unstimulated whole saliva after treatment with resin‐based composite fillings in vivo
Author(s) -
Michelsen Vibeke B.,
Kopperud Hilde B. M.,
Lygre Gunvor B.,
Björkman Lars,
Jensen Einar,
Kleven Inger S.,
Svahn Johanna,
Lygre Henning
Publication year - 2012
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.2011.00897.x
Subject(s) - saliva , methacrylate , monomer , (hydroxyethyl)methacrylate , chemistry , dental composite , chromatography , bisphenol a , composite number , nuclear chemistry , materials science , epoxy , composite material , polymer , organic chemistry , biochemistry
Michelsen VB, Kopperud HBM, Lygre GB, Björkman L, Jensen E, Kleven IS, Svahn J, Lygre H. Detection and quantification of monomers in unstimulated whole saliva after treatment with resin‐based composite fillings in vivo.
Eur J Oral Sci 2012; 120: 89–95. © 2012 Eur J Oral Sci Resin‐based dental restorative materials contain allergenic methacrylate monomers, which may be released into saliva after restorative treatment. Monomers from resin‐based composite materials have been demonstrated in saliva in vitro ; however, studies analyzing saliva after restorative therapy are scarce. The aim of this study was to quantify methacrylate monomers in saliva after treatment with a resin‐based composite filling material. Saliva was collected from 10 patients at four start points – before treatment, and 10 min, 24 h, and 7 d after treatment – and analysed by combined chromatography/mass spectrometry. The monomers bisphenol‐A diglycidyl methacrylate (Bis‐GMA), 2‐hydroxyethyl methacrylate (HEMA), and urethane dimethacrylate (UDMA) were detected and quantified in the samples collected shortly (10 min) after treatment. The amounts detected ranged from 0.028 to 9.65 μg ml −1 for Bis‐GMA, from 0.015 to 0.19 μg ml −1 for HEMA, and from 0.004 to 1.2 μg ml −1 for UDMA. Triethyleneglycol dimethacrylate (TEGDMA) was detected in four of the samples. Ethoxylated bisphenol‐A dimethacrylate (Bis‐EMA) was not detected. Monomers were not detected in saliva samples collected before treatment, or 24 h or 7 d after treatment, with the exception of one sample, 24 h after treatment, in which HEMA was detected. In conclusion, monomers from the investigated resin‐based composite and adhesive system were present in saliva shortly after treatment. One week after treatment, no monomers could be detected in patients’ saliva samples.