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Relaxin is up‐regulated in the rat ovary by orthodontic tooth movement
Author(s) -
Yang SoYoung,
Ko HyunMi,
Kang JeeHae,
Moon YeonHee,
Yoo HongIl,
Jung NaRi,
Kim MinSeok,
Cho JinHyung,
Oh WonMann,
Kim SunHun
Publication year - 2011
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.2011.00814.x
Subject(s) - relaxin , ovary , blot , periodontal fiber , molar , connective tissue , endocrinology , immunofluorescence , corpus luteum , medicine , chemistry , hormone , dentistry , antibody , pathology , biochemistry , immunology , gene
Yang S‐Y, Ko H‐M, Kang J‐H, Moon Y‐H, Yoo H‐I, Jung N‐R, Kim M‐S, Cho J‐H, Oh W‐M, Kim S‐H. Relaxin is up‐regulated in the rat ovary by orthodontic tooth movement.
Eur J Oral Sci 2011; 119: 115–120. © 2011 Eur J Oral Sci Relaxin (Rln) is an ovarian hormone that stimulates osteoclastic and osteoblastic activities and connective tissue turnover. To investigate the expression of Rln during orthodontic tooth movement, rats were implanted with orthodontic appliances that connected a spring from the upper incisors to the first molar with a 70 cN force. Rats in each group were killed 6, 48, and 144 h after activating the appliance, and the levels of Rln1 and Rln3 expression in the ovary were determined by real‐time RT‐PCR, northern blots, western blots, and immunofluorescence analyses. The amount of tooth movement induced by the orthodontic force increased in a time‐dependent manner. The levels of Rln1 mRNA increased by 12‐, 41‐, and 263‐fold at 6, 48, and 144 h, respectively, after orthodontic tooth movement. The time‐dependent increase in the concentration of Rln 1 protein in the ovary was also confirmed by western blotting. Rln 1 was localized in the granulosa cells of the ovarian follicles, and the immunoreactivity against Rln 1 was increased by the movement. In contrast, the concentration of Rln 3 was below the level of detection. The results of this study suggest that local changes in periodontal tissues induced by orthodontic tooth movement may affect Rln1 expression in the ovary. However, further studies are needed to decipher the mechanisms involved and the possible contribution of the increased level of expression of Rln 1 to the tooth movement.