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Enzymatic degradation of adhesive–dentin interfaces produced by mild self‐etch adhesives
Author(s) -
De Munck Jan,
Mine Atsushi,
Van den Steen Philippe E.,
Van Landuyt Kirsten L.,
Poitevin André,
Opdenakker Ghislain,
Van Meerbeek Bart
Publication year - 2010
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.2010.00758.x
Subject(s) - adhesive , dentin , materials science , ultimate tensile strength , degradation (telecommunications) , matrix metalloproteinase , bond strength , composite material , collagen fibril , dental bonding , dentistry , chemistry , layer (electronics) , biophysics , biochemistry , medicine , telecommunications , computer science , biology
De Munck J, Mine A, Van den Steen PE, Van Landuyt KL, Poitevin A, Opdenakker G, Van Meerbeek B. Enzymatic degradation of adhesive–dentin interfaces produced by mild self‐etch adhesives.
Eur J Oral Sci 2010; 118: 494–501. © 2010 Eur J Oral Sci Endogenous matrix metalloproteinases (MMPs) released by adhesive procedures may degrade collagen in the hybrid layer and so compromise the bonding effectiveness of etch‐and‐rinse adhesives. In this study, endogenous enzymatic degradation was evaluated for several simplified self‐etch adhesives. In addition, primers were modified by adding two MMP inhibitors: chlorhexidine, a commonly used disinfectant, but also a non‐specific MMP inhibitor; and SB‐3CT, a specific inhibitor of MMP‐2 and MMP‐9. Gelatin zymography of fresh human dentin powder was used to identify the enzymes released by the adhesives. Micro‐tensile bond strength (μTBS) testing was used to assess the mechanical properties of resin–dentin interfaces over time. In none of the experimental groups treated with the mild self‐etch adhesives was MMP‐2 and/or MMP‐9 identified. Also, no difference in the μTBS was measured for the inhibitor‐modified and the control inhibitor‐free adhesives after 6 months of water storage. It is concluded that in contrast to etch‐and‐rinse adhesives, the involvement of endogenous MMP‐2 and MMP‐9 in the bond‐degradation process is minimal for mild self‐etch adhesives.