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Intracellular Ca 2+ responses and cell volume regulation upon cholinergic and purinergic stimulation in an immortalized salivary cell line
Author(s) -
Aure Marit H.,
Røed Asbjørn,
Galtung Hilde Kanli
Publication year - 2010
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.2010.00738.x
Subject(s) - purinergic receptor , carbachol , intracellular , immortalised cell line , cholinergic , stimulation , endocrinology , medicine , chemistry , microbiology and biotechnology , transient receptor potential channel , trpv4 , cell culture , acetylcholine , extracellular , biology , cell , receptor , biochemistry , genetics
Aure MH, Røed A, Kanli Galtung H. Intracellular Ca2+responses and cell volume regulation upon cholinergic and purinergic stimulation in an immortalized salivary cell line. Eur J Oral Sci 2010; 118: 237–244. © 2010 The Authors. Journal compilation © 2010 Eur J Oral Sci The water channel aquaporin 5 (AQP5) seems to play a key role in salivary fluid secretion and appears to be critical in the cell volume regulation of acinar cells. Recently, the cation channel transient potential vanilloid receptor 4 (TRPV4) was shown to be functionally connected to AQP5 and also to cell volume regulation in salivary glands. We used the Simian virus 40 (SV40) immortalized cell line SMG C10 from the rat submandibular salivary gland to investigate the effect of ATP and the neurotransmitter analogue carbachol on Ca 2+ signalling and cell volume regulation, as well as the involvement of TRPV4 in the responses. We used fura‐2‐AM imaging, cell volume measurements, and western blotting. Both carbachol and ATP increased the concentration of intracellular Ca 2+ , but no volume changes could be measured. Inhibition of TRPV4 with ruthenium red impaired both ATP‐ and carbachol‐stimulated Ca 2+ signals. Peak Ca 2+ signalling during hyposmotic exposure was significantly decreased following inhibition of TRPV4, while the cells’ ability to volume regulate appeared to be unaffected. These results show that in the SMG C10 cells, simulation of nervous stimulation did not induce cell swelling, although the cells had intact volume regulatory mechanisms. Furthermore, even though Ca 2+ signals were not needed for this volume regulation, TRPV4 seems to play a role during ATP and carbachol stimulation.