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Applying an excessive mechanical stress alters the effect of subchondral osteoblasts on chondrocytes in a co‐culture system
Author(s) -
Lin YuYu,
Tanaka Nobuaki,
Ohkuma Satoru,
Iwabuchi Yasunori,
Tanne Yuki,
Kamiya Takashi,
Kunimatsu Ryo,
Huang YuChing,
Yoshioka Motoko,
Mitsuyoshi Tomomi,
Tanimoto Kotaro,
Tanaka Eiji,
Tanne Kazuo
Publication year - 2010
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.2010.00710.x
Subject(s) - bone sialoprotein , aggrecan , chemistry , osteoarthritis , cartilage , chondrocyte , microbiology and biotechnology , type ii collagen , endochondral ossification , bone remodeling , type i collagen , matrix (chemical analysis) , osteoblast , endocrinology , anatomy , in vitro , osteocalcin , pathology , biology , alkaline phosphatase , biochemistry , medicine , alternative medicine , chromatography , enzyme , articular cartilage
Lin Y‐Y, Tanaka N, Ohkuma S, Iwabuchi Y, Tanne Y, Kamiya T, Kunimatsu R, Huang Y‐C, Yoshioka M, Mitsuyoshi T, Tanimoto K, Tanaka E, Tanne K. Applying an excessive mechanical stress alters the effect of subchondral osteoblasts on chondrocytes in a co‐culture system. Eur J Oral Sci 2010; 118: 151–158. © 2010 The Authors. Journal compilation © 2010 Eur J Oral Sci Osteoarthritis (OA) sometimes occurs as a consequence of repeated microtrauma involved in parafunction, which may lead to microfracture in the subchondral bone. The aim of this in vitro study was to evaluate the effects of subchondral osteoblasts in loading with repeated excessive mechanical stress on the metabolism of overlying chondrocytes. A high‐magnitude cyclic tensile stress of 15 kPa (30 cycles min −1 ) was applied to the cultured osteoblasts obtained from porcine mandibular condyles. The chondrocytes in alginate beads were then co‐cultured with mechanically stressed or unstressed osteoblasts. Chondrocytes co‐cultured with unstressed osteoblasts showed a phenotypic shift to hypertrophic chondrocytes, characterized by decreased expression of type II collagen, aggrecan , Sry‐related HMG box (SOX‐9) , and cartilage oligomeric matrix protein (COMP) genes and increased expression of type X collagen and bone sialoprotein ( BSP ) genes, suggesting that the co‐culture may change the chondrocyte differentiation to some extent. These changes were more distinct in chondrocytes co‐cultured with excessively mechanically stressed osteoblasts. After co‐culture with stressed osteoblasts, the expressions of matrix metalloproteinase ( MMP)1 , MMP3 and MMP13 genes were also enhanced and the synthesis of DNA, proteoglycan and collagen were significantly decreased in chondrocytes. These results demonstrate that alterations in cartilage metabolism can be induced by stressed osteoblasts, indicating a possible explanation for the onset and progression of OA.