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Evaluation of the antimicrobial effect of photodynamic antimicrobial therapy in an in situ model of dentine caries
Author(s) -
Lima Juliana P. M.,
Sampaio de Melo Mary A.,
Borges Fátima M. C.,
Teixeira Alrieta H.,
SteinerOliveira Carolina,
Nobre dos Santos Marinês,
Rodrigues Lidiany K. A.,
Zanin Iriana C. J.
Publication year - 2009
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.2009.00662.x
Subject(s) - antimicrobial , streptococcus mutans , photodynamic therapy , pact , chemistry , dentistry , microorganism , bacteria , microbiology and biotechnology , medicine , biology , history , genetics , organic chemistry , archaeology
Photodynamic antimicrobial therapy (PACT) promotes bacterial death as a result of the photosensitization of microbial components. This study evaluated the effect of PACT on dentine caries produced in situ . Over the course of 14 d, 20 volunteers wore intra‐oral devices containing human dentine slabs that were treated 10 times daily with a 40% sucrose solution. Afterwards, the antimicrobial effect of toluidine blue O, associated with 47 or 94 J cm −2 of a light‐emitting diode, was evaluated. Before and after the treatments, dentine samples were analysed with regard to the total number of microorganisms, total streptococci, mutans streptococci, and lactobacilli. Significant reductions in the bacterial count were observed for PACT with both energy densities tested, with the following values observed for 47 and 94 J cm −2 of irradiation: for total streptococci, 3.45 and 5.18; for mutans streptococci, 3.08 and 4.16; for lactobacilli, 3.24 and 4.66; and for total microorganisms, 4.29 and 5.43, respectively. The control, treated with 94 J cm −2 of irradiation alone, was also effective against all bacteria. To conclude, PACT was effective in killing oral microorganisms present in dentine caries produced in situ and may be a useful technique for eliminating bacteria from dentine carious lesions before restoration.