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The location and characteristics of two populations of dental pulp cells affect tooth development
Author(s) -
Sumita Yoshinori,
Tsuchiya Shuhei,
Asahina Izumi,
Kagami Hideaki,
Honda Masaki J.
Publication year - 2009
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.2008.00603.x
Subject(s) - dentistry , affect (linguistics) , pulp (tooth) , orthodontics , medicine , psychology , communication
This study investigated the characteristics of two dental pulp cell populations during the early stages of crown formation in porcine teeth. A transplantation method was developed to reproduce epithelial cell–mesenchymal cell interactions during odontogenesis (tooth development). The technique allowed two types of cells/tissue to be combined in vivo . Populations of cells localized in the cervical loop epithelium region, dental pulp horn, and dental pulp core chambers were isolated and dissociated into single cells. Each population was examined for its gene‐expression pattern using both semiquantitative and quantitative reverse transcription–polymerase chain reaction (RT‐PCR) analyses, and for its tissue‐formation capability by combining the cervical loop epithelial cells with either pulp horn cells or pulp core cells on biodegradable collagen scaffolds that were subsequently examined using histology and immunohistology. Gene‐expression patterns showed that pulp horn cells were more mature than pulp core cells. Cervical loop epithelial cells combined with pulp horn cells mainly reconstituted dentin–cementum structures. By contrast, cervical loop epithelial cells combined with pulp core cells reconstituted enamel–dentin structures. These results suggest that mesenchymal cells residing in a specific location of the pulp possess a specific tissue‐formation potential when combined with epithelial cells.