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Effect of 2‐hydroxyethyl‐methacrylate (HEMA) on the phagocytic and respiratory burst activity of human neutrophils and monocytes
Author(s) -
Andersson Jennie,
Dahlgren Ulf I.
Publication year - 2008
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.2008.00541.x
Subject(s) - respiratory burst , phagocytosis , propidium iodide , chemistry , flow cytometry , immunology , respiratory system , neutrophil extracellular traps , methacrylate , monocyte , rhodamine 123 , granulocyte , medicine , inflammation , biochemistry , programmed cell death , apoptosis , monomer , organic chemistry , antibiotics , multiple drug resistance , polymer
Neutrophils and monocytes/macrophages (MØ), found in oral mucosa and gingival sulcus, phagocytose and kill bacteria using products produced during a respiratory burst. 2‐Hydroxyethyl‐methacrylate (HEMA) is a major component released from resin glass ionomer and dental adhesives. Hence, in pulp and gingiva, phagocytes can come into contact with unpolymerized HEMA monomers. The aim of this study was to examine the effects of exposure to HEMA on neutrophil and monocyte bactericidal function. Blood collected from five female volunteers was exposed in vitro to HEMA for 2 h and then phagocytosis, respiratory burst, and cellular integrity were measured using flow cytometry. Respiratory burst was quantified by measuring fluorescent rhodamine 123 generated via oxidation of dihydrorhodamine 123. Cellular membrane integrity was evaluated by staining with propidium iodide. The respiratory burst activity of the neutrophils was significantly decreased by exposure to 7.5 and 15 mM HEMA. No significant effect of HEMA was seen on the number of granulocytes or monocytes capable of performing respiratory burst. Furthermore, there was no significant effect of HEMA on the phagocytic activity of the monocytes or the granulocytes. In conclusion, HEMA did not affect the phagocytosis activity of neutrophils; however, the ability of the cells to kill internalized prey was significantly reduced.