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Distribution of SIBLING proteins in the organic and inorganic phases of rat dentin and bone
Author(s) -
Huang Bingzhen,
Sun Yao,
Maciejewska Izabela,
Qin Disheng,
Peng Tao,
McIntyre Bradley,
Wygant James,
Butler William T,
Qin Chunlin
Publication year - 2008
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.2008.00522.x
Subject(s) - dmp1 , bone sialoprotein , dentin , chemistry , dentin sialophosphoprotein , osteopontin , gel electrophoresis , dentinogenesis , microbiology and biotechnology , chromatography , biochemistry , osteocalcin , odontoblast , biology , dentistry , viral matrix protein , gene , alkaline phosphatase , medicine , immunology , enzyme
The SIBLING protein family is a group of non‐collagenous proteins (NCPs) that includes dentin sialophosphoprotein (DSPP), dentin matrix protein 1 (DMP1), bone sialoprotein (BSP), and osteopontin (OPN). In the present study, we compared these four proteins in different phases of rat dentin and bone. First, we extracted NCPs in the unmineralized matrices and cellular compartments using guanidium‐HCl (G1). Second, we extracted NCPs closely associated with hydroxyapatite using an EDTA solution (E). Last, we extracted the remaining NCPs again with guanidium‐HCl (G2). Each fraction of Q‐Sepharose ion‐exchange chromatography was analyzed using sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE), Stains‐All stain, and with western immunoblotting. In dentin, the NH 2 ‐terminal fragment of DSPP and its proteoglycan form were primarily present in the G1 extract, whereas the COOH‐terminal fragment of DSPP was present exclusively in the E extract. The processed NH 2 ‐terminal fragment of DMP1 was present in G1 and E extracts, whereas the COOH‐terminal fragment of DMP1 existed mainly in the E extract. Bone sialoprotein was present in all three extracts of dentin and bone, whereas OPN was present only in the G1 and E extracts of bone. The difference in the distribution of the SIBLING proteins between organic and inorganic phases supports the belief that these molecular species play different roles in dentinogenesis and osteogenesis.