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Effect of inhibition of inducible nitric oxide synthase (iNOS) on the murine splenic immune response induced by Aggregatibacter ( Actinobacillus ) actinomycetemcomitans lipopolysaccharide
Author(s) -
Sosroseno W.,
Musa M.,
Ravichandran M.,
Ibrahim M. F.,
Bird P. S.,
Seymour G. J.
Publication year - 2008
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.2007.00501.x
Subject(s) - lipopolysaccharide , nitric oxide synthase , immune system , aggregatibacter actinomycetemcomitans , nitric oxide , spleen , interferon gamma , omega n methylarginine , immunology , chemistry , microbiology and biotechnology , biology , porphyromonas gingivalis , endocrinology , medicine , periodontitis
Animal studies suggest that inducible nitric oxide synthase (iNOS) may be associated with destructive periodontal disease. l‐ N 6 ‐(1‐Iminoethyl)‐lysine ( l ‐NIL) has been shown to inhibit iNOS in a selective manner, and hence the aim of the present study was to test the hypothesis that treatment with l ‐NIL may induce a T‐cell helper 1 (Th1)‐like immune response by Aggregatibacter ( Actinobacillus ) actinomycetemcomitans lipopolysaccharide (LPS)‐stimulated murine spleen cells in vitro . BALB/c mice were either sham‐immunized or immunized with A. actinomycetemcomitans LPS. Spleen cells were stimulated with A. actinomycetemcomitans LPS in the presence or absence of l ‐NIL. Nitric oxide (NO), iNOS activity, specific IgG subclass antibodies, interferon‐ γ (IFN‐ γ ), and interleukin‐4 (IL‐4) levels and cell proliferation were determined. The results showed that treatment with l ‐NIL suppressed both NO production and iNOS activity but enhanced specific IgG2a, IFN‐ γ levels, and increased cell proliferation following stimulation with A. actinomycetemcomitans LPS‐stimulated cells. The results of the present study suggest that inhibition of iNOS activity by l ‐NIL may skew the A. actinomycetemcomitans LPS‐stimulated murine splenic immune response towards the Th1‐like immune profile in vitro .