Premium
The 120 kDa cell‐binding fragment of fibronectin up‐regulates migration of αvβ6‐expressing cells by increasing matrix metalloproteinase‐2 and ‐9 secretion
Author(s) -
AlHazmi Nadia,
Thomas Gareth J.,
Speight Paul M.,
Whawell Simon A.
Publication year - 2007
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.2007.00481.x
Subject(s) - fibronectin , matrix metalloproteinase , extracellular matrix , secretion , integrin , metalloproteinase , motility , cell migration , microbiology and biotechnology , chemistry , cell culture , wound healing , cell , biology , biochemistry , immunology , genetics
Changes in the extracellular matrix, integrin expression, and protease secretion occur in wound healing and cancer and these systems are thought to play a crucial role in such processes. In this study, experiments were performed to examine the interaction of epithelial cells with the 120 kDa cell‐binding fibronectin fragment. Cell migration was significantly increased in response to the 120 kDa fragment when compared with the full‐length molecule, but only in cells overexpressing the beta‐6 integrin (VB6). This involved the up‐regulation of matrix metalloproteinases (MMPs) 2 and 9, the levels of which are increased in the supernatant from VB6 cells plated on the 120 kDa fragment. Inhibition of MMP activity with both tissue inhibitor of metalloproteinase‐1 (TIMP‐1) and a chemical inhibitor (Ro32‐3580) resulted in a significant reduction in migration on the fibronectin fragment. In vitro this fragment can be generated by incubating full‐length fibronectin with purified MMP‐9.Expression of the β6 integrin subunit, which is only seen in wound healing and cancer, enhances the motility of and MMP secretion by epithelial cells in response to the 120 kDa fibronectin fragment. These enzymes can then further degrade fibronectin to generate additional fragments, leading to the creation of a positive feedback loop, which may be of significance in disease processes.