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Changes in matrix phosphorylation during bovine dentin development
Author(s) -
Verdelis Kostas,
Lukashova Lyudmilla,
Yamauchi Mitsuo,
Atsawasuwan Peter,
Wright John T.,
Peterson Margaret G. E.,
Jha Divya,
Boskey Adele L.
Publication year - 2007
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.2007.00463.x
Subject(s) - dentin , mineralization (soil science) , chemistry , mineralized tissues , phosphorylation , phosphate , matrix (chemical analysis) , biochemistry , microbiology and biotechnology , dentistry , biology , chromatography , medicine , organic chemistry , nitrogen
Phosphorylation of the organic matrix proteins of dentin is important for the initiation of mineralization, but its relevance in later mineralization stages is controversial. The objective of this study was to analyze changes in the total matrix phosphate content during dentin development and to identify their origin. Amino acid and total matrix phosphate analyses of microdissected developing mantle and circumpulpal fetal bovine dentin specimens were performed. The amino acid composition showed few changes during mantle and circumpulpal dentin maturation. However, the total matrix phosphate content showed a significant, positive correlation with tissue maturation in both mantle and circumpulpal dentin, with a two‐ and a three‐fold increase, respectively, being observed. The data indicate that changes occur in the pattern of phosphorylation of matrix proteins during dentin maturation, which we suggest may play a functional role in later stages of tooth mineralization.