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Differences between single‐ and dual‐species biofilms of Streptococcus mutans and Veillonella parvula in growth, acidogenicity and susceptibility to chlorhexidine
Author(s) -
Kara Duygu,
Luppens Suzanne B. I.,
Cate Jacob M.
Publication year - 2006
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.2006.00262.x
Subject(s) - biofilm , streptococcus mutans , microbiology and biotechnology , veillonella , chlorhexidine , chemistry , bacteria , dental plaque , biology , streptococcus , dentistry , medicine , genetics
Streptococcus mutans, considered a primary pathogen in dental caries, thrives in dental plaque, which is a multispecies biofilm. Metabolic interactions between S. mutans and Veillonella parvula have been suggested. In this study we developed a biofilm model to quantify single‐species ( S. mutans or V. parvula ) and dual‐species ( S. mutans and V. parvula ) biofilm formation, and we identified the differences between the respective biofilms in terms of growth, acid formation, and response to chlorhexidine. Polystyrene 96‐well microtiter plates were used for biofilm formation. These biofilms were exposed to various chlorhexidine concentrations (0.025–0.4 mg ml −1 ) and treatment conditions. Growth of the biofilms and the effects of chlorhexidine were evaluated by viable counts. Viability of the two species in all biofilm types was similar (≈ 10 8 colony‐forming units per well) after 72 h. Lactic acid accumulation of dual‐species biofilms was significantly lower at 48 and 72 h than single‐species biofilms of S. mutans . Dual‐species biofilms were less susceptible to chlorhexidine than single‐species biofilms when a neutralization step was included. These results indicate that bacteria in dual‐species biofilms have different properties from bacteria in single‐species biofilms.