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Dynamic protein expression patterns during intraoral wound healing in the rat
Author(s) -
Van Beurden Hugo E.,
Snoek Patricia A. M.,
Von den Hoff Johannes W.,
Torensma Ruurd,
Maltha Jaap C.,
KuijpersJagtman Anne M.
Publication year - 2005
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.2005.00200.x
Subject(s) - wound healing , fibroblast , vinculin , vimentin , phenotype , microbiology and biotechnology , pathology , medicine , biology , in vitro , immunology , focal adhesion , immunohistochemistry , signal transduction , genetics , gene
Wound healing after cleft palate surgery is often associated with impairment of maxillary growth and dento‐alveolar development. Wound contraction and scar tissue formation contribute strongly to these effects. In vitro studies have revealed that fibroblasts isolated during different phases of palatal wound healing show phenotypical differences. They change from a quiescent to an activated state and then partly back to a quiescent state. In this study, we evaluated the existence of fibroblast phenotypes at several time‐points during palatal wound healing in the rat. Based on cytoskeletal changes ( α ‐sma, vimentin, vinculin), integrin expression ( α 1 , α 2 , α v and β 1 ) and changes in cellularity, we conclude that phenotypically different fibroblast populations are also present during in vivo wound healing. α ‐sma and the integrin subunits α 1 and α v were significantly up‐regulated, and vinculin was significantly down‐regulated, at early time‐points compared to late time‐points in wound healing. These changes point to an activated fibroblast state early in wound healing. Later in wound healing, these activated fibroblasts return only partially to the unwounded situation. These results strongly support the idea that different fibroblast populations with specific phenotypes occur in the course of palatal wound healing.