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Single species biofilm‐forming ability of root canal isolates on gutta‐percha points
Author(s) -
Takemura Naoki,
Noiri Yuichiro,
Ehara Atsushi,
Kawahara Takashi,
Noguchi Nobuo,
Ebisu Shigeyuki
Publication year - 2004
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.2004.00165.x
Subject(s) - microbiology and biotechnology , biofilm , enterococcus faecalis , fusobacterium nucleatum , root canal , prevotella intermedia , staphylococcus aureus , biology , porphyromonas gingivalis , chemistry , bacteria , medicine , dentistry , genetics
The participation of bacterial biofilms in the over‐filled gutta‐percha points associated with refractory periapical periodontitis has recently been reported. This study investigated the initial biofilm‐forming ability of root canal isolates ( Enterococcus faecalis, Streptococcus sanguis , Strep. intermedius , Strep. pyogenes , Staphylococcus aureus , Fusobacterium nucleatum , Propionibacterium acnes , Porphyromonas gingivalis and Prevotella intermedia ) on gutta‐percha points in vitro . Each bacterial strain was suspended in 100% cell culture medium or in culture medium containing 4.5, 45 or 90% (vol/vol) serum. The bacterial suspensions were then co‐incubated anaerobically with gutta‐percha points for 7 d. The gutta‐percha points were processed for scanning electron microscopic observation and examined for biofilm presence and thickness. E. faecalis, Strep. sanguis , Strep. intermedius , Strep. pyogenes and Staph. aureus biofilms were generated on the surfaces of the specimens incubated in culture medium supplemented with 45 or 90% (vol/vol) serum. The E. faecalis and Strep. sanguis biofilms were significantly thicker than those of Strep. intermedius , Strep. pyogenes and Staph. aureus . No biofilms were detected on the specimens incubated with F. nucleatum , Prop. acnes , Porph. gingivalis and Prev. intermedia . These findings suggest that Gram‐positive facultative anaerobes have the ability to colonize and form extracellular matrices on gutta‐percha points, while serum plays a crucial role in biofilm formation.