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Cytotoxicity and sealing properties of four classes of endodontic sealers evaluated by succinic dehydrogenase activity and confocal laser scanning microscopy
Author(s) -
Bouillaguet Serge,
Wataha John C.,
Lockwood Petra E.,
Galgano Camillo,
Golay Antoine,
Krejci Ivo
Publication year - 2004
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.2004.00115.x
Subject(s) - confocal laser scanning microscopy , confocal , laser microscopy , chemistry , scanning electron microscope , confocal laser scanning microscope , cytotoxicity , dentistry , succinic dehydrogenase , laser , laser scanning , confocal microscopy , biomedical engineering , materials science , medicine , biochemistry , biology , composite material , enzyme , microbiology and biotechnology , optics , in vitro , physics
The objectives of this study were to evaluate the cytotoxicity and sealing properties of four classes of endodontic sealers (PCS/Kerr, RoekoSeal/Roeko, TopSeal/Dentsply, and EndoREZ/Ultradent). For cytotoxicity testing (MTT method), the materials were either placed immediately in contact with cultured cells or 24 h after setting, then evaluated at three subsequent time points (24 h, 48 h, or 1 wk). For the leakage study, extracted human roots were obturated with acrylic cones and sealers and immersed for 48 h into rhodamine‐labeled lipopolysaccharide. The roots were then observed under a confocal laser scanning microscope to estimate (semiquantitatively) the presence of the rhodamine‐lipopolysaccharide (LPS) inside the canal. The results showed that cytotoxicity generally increased with time, and that most materials pose significant cytotoxic risks, particularly in the freshly mixed condition. Further, all materials showed significant leakage although there was large variation among teeth. Overall, the silicon‐based material (Roeko Seal) was less cytotoxic and more effective in sealing root canals against LPS leakage than other materials.