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Effects of aFGF, bFGF, TGFβ1 and IGF‐I on odontoblast differentiation in vitro
Author(s) -
Martín Amaya,
Unda FernandoJosé,
BègueKirn Catherine,
Ruch JeanVictor,
Aréchaga Juan
Publication year - 1998
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.1998.tb02162.x
Subject(s) - odontoblast , extracellular matrix , chemistry , microbiology and biotechnology , basic fibroblast growth factor , biology , growth factor , pathology , biochemistry , medicine , pulp (tooth) , receptor
In this work, we investigated the effects of aFGF and bFGF alone or combined with TGFβ1 or IGF‐I on odontoblast differentiation. Trypsin‐isolated dental papillae from day 17 mandibular first molar were cultured in semisolid‐agar medium for 6 d. Our results demonstrated that aFGF, bFGF or combinations of these promoted cell polarization at the periphery of the dental Papillae. Moreover, simultaneous addition of aFGF and TGFβ1 to dental Papillae cultures induced both polarization and functional differentiation of odontoblast‐like cells, as well as extracellular matrix deposition. Combination of aFGF or bFGF with IGF‐I caused cell polarization at the surface of dental papillae, but matrix secretion was restricted to a few explants. In the presence of bFGF and TGFβ1, the explants had pronounced cell elongations but no matrix deposition. These results indicate that aFGF or bFGF is not able to induce odontoblast differentiation alone. However, both aFGF and bFGF can act synergistically with TGFβ1 and IGF‐I to strengthen their inductive effects and promote gradients of cytological and functional changes in odontoblast‐like cells.