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EGF does not induce Msx‐1 and Msx‐2 in dental mesenchyme
Author(s) -
Wang YuHsiung,
Kollar Edward J.,
Upholt William B.,
Mina Mina
Publication year - 1998
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.1998.tb02159.x
Subject(s) - mesenchyme , dental lamina , in situ hybridization , epithelium , molar , microbiology and biotechnology , biology , chemistry , mesenchymal stem cell , anatomy , gene expression , pathology , gene , biochemistry , genetics , medicine , paleontology , odontogenic
Previous heterospecific tissue recombinations indicate that mandibular epithelium exerts the first known inductive signal for odontogenesis in mouse embryos. BMP‐4 and EGF are two growth factors implicated as signaling molecules mediating the initial inductive epithelial‐mesenchymal interactions during odontogenesis. The purpose of the present study was to examine and compare the effects of these growth factors and mouse mandibular epithelium on expression of Msx‐1 and Msx‐2 genes in molar‐forming mesenchyme. Agarose beads soaked in growth factors or pieces of mouse mandibular epithelium (E11) were placed in contact with E11 molar‐forming mesenchyme and cultured for 24 h. Whole‐mount in situ hybridization analysis revealed that, in contrast to mouse mandibular epithelium and BMP‐4‐releasing beads, EGF‐releasing beads did not induce the expression of Msx‐1 and Msx‐2 in E11 molar‐forming mesenchyme. These observations suggest that whereas BMP‐4 may be involved in activation of Msx‐1 and Msx‐2 in the underlying mesenchyme, EGF may regulate events involved in the formation of dental lamina.