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Chondroitin sulfate isomers in synovial fluid of healthy and diseased human temporomandibular joints
Author(s) -
Okazaki Joji,
Kakudo Kenji,
Kamada Aiko,
Utoh Eigo,
Gonda Yoshimichi,
Shirasu Rikiya,
Sakaki Tetsuya
Publication year - 1997
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.1997.tb02141.x
Subject(s) - synovial fluid , chondroitin sulfate , chemistry , chondroitin , temporomandibular joint , glycosaminoglycan , anatomy , chromatography , biochemistry , pathology , osteoarthritis , medicine , alternative medicine
Synovial fluid was collected from the superior articular cavity of the temporomandibular joint in patients with unilateral internal derangement and joint pain whose contralateral joint was healthy. Glycosaminoglycans were liberated by digestion with pronase E, and precipitated with cetylpyridinium chloride and ethanol. Unsaturated disaccharide isomers of chondroitin sulfate, obtained following chondroitinase ACII digestion, were analyzed by high‐performance liquid chromatography. Analytic data indicated that ΔDi‐0S and ΔDi‐6S were often found in chondroitin sulfate from the fluid of the diseased joints. The amounts of ΔDi‐0S and ΔDi‐6S differed significantly between synovial fluid samples from the diseased and healthy joints. Comparison of the relative proportions of the unsaturated disaccharides in the synovial fluid with previously reported values for several tissues, indicated that the chondroitin sulfate originated from articular cartilage, with possibly some contributions from soft connective tissues and serum present in the synovial fluid. These results suggest that chondroitin sulfate in the synovial fluid provides a useful indicator of the degree of internal derangement of the temporomandibular joint.