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Dentin bonding: effect of tubule orientation on hybrid‐layer formation
Author(s) -
Schüpbach Peter,
Krejci Ivo,
Lutz Felix
Publication year - 1997
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.1997.tb00251.x
Subject(s) - dentinal tubule , dentin , tubule , smear layer , transmission electron microscopy , materials science , layer (electronics) , confocal , scanning electron microscope , pulp (tooth) , chemistry , composite material , dentistry , nanotechnology , optics , medicine , physics , kidney , endocrinology
Schüpbach P. Krejci I, Felix L: Dentin bonding: effect of tubule orientation on hybrid‐layer formation. In an attempt to compare the morphology of the resin‐dentin interface in areas where the dentinal tubules run perpendicularly or at an angle to the cavity surface with that of areas where they run parallel to it, we studied a dentin adhesive system using transmission electron microscopy and fluorescence confocal laser scanning microscopy. The design of the study included the simulation of the normal hydrostatic pressure within the pulp and the dentinal tubules. Following acid etching of the dentinal surface with maleic acid/HEMA, the smear layer was removed, and a superficial zone was demineralized in such a way that the exposed collagenous dentin matrix retained its integrity. Confocal laser scanning microscopal investigations using primer labeled with rhodamine B showed that the penetration of the primer occurred not only vertically via surface porosities, but mainly laterally, via the dentinal tubules. The adhesive resin labeled with fluorescein completely infiltrated the demineralized layer, thereby forming a hybrid layer. The orientation of the dentinal tubules had a profound effect on the formation of the hybrid layer. In areas with perpendicular tubule orientation, the layer was 3.2 ± 0.8 μm thick, showing solid 27.2 ± 0.8 μm long resin tags in the dentinal tubules, and a network of tiny tags in their side‐branches. In areas with parallel tubule orientation, the layer was significantly thinner (1.3 ± 0.6 μm) and resin tags were absent.