Premium
Expression of BMP‐2 and TGF‐β1 mRNA during healing of the rabbit mandible
Author(s) -
Si Xiaohui,
Jin Yan,
Yang Lianjia,
Tipoe Geirge L.,
White Frank H.
Publication year - 1997
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.1997.tb00248.x
Subject(s) - intramembranous ossification , chondrogenesis , bone healing , bone morphogenetic protein 2 , mesenchymal stem cell , in situ hybridization , microbiology and biotechnology , chemistry , bone morphogenetic protein , transforming growth factor , endochondral ossification , messenger rna , biology , anatomy , cartilage , gene , biochemistry , in vitro
To identify the cell types which produce BMP and TGF‐β during fracture healing and to elucidate the interactions between BMP and TGF‐β in regulating cell proliferation and differeentiation at various stages, an experimental model of fracture healing in the rabbit mandible was established and the expression of BMP‐2 and TGF‐β1 mRNA was studied at different healing stages by in situ hybridization. The results showed that undifferentiated mesenchymal cells, differentiating osteoblasts and chondroblasts, had higher levels of BMP‐2 mRNA at the stage of intramembranous bone formation and early chondrogenesis, while the level of TGF‐β1 mRNA expression was closely associated with the active synthetic stage of osteoblasts and chondrocytes. These obserbvations suggest that both BMP and TGF‐β are involved in the regulation of fracture healing, BMP may play an important role in bone induction and early chondrogenesis, while TGF‐β regulates the proliferation and active synthetic ability of chondrocytes and osteoblasts.