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Keratinocyte growth factor mRNA expression in periodontal ligament fibroblasts
Author(s) -
Dabelsteen Sally,
Wandall Hans H.,
Grøn Birgitte,
Dabelsteen Erik
Publication year - 1997
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.1997.tb00223.x
Subject(s) - periodontal fiber , keratinocyte growth factor , fibroblast , connective tissue , messenger rna , microbiology and biotechnology , growth factor , keratinocyte , chemistry , fetal bovine serum , biology , cell culture , andrology , cell , dentistry , medicine , biochemistry , gene , genetics , receptor
Keratinocyte growth factor (KGF) is a fibroblast growth factor which mediates epithelial growth and differentiation. K.GF is expressed in subepithelial fibroblasts, but generally not in fibroblasts of deep connective tissue, such as fascia and ligaments. Here we demonstrate that KGF mRNA is expressed in periodontal ligament fibroblasts, and that the expression is increased upon serum stimulation. Fibroblasts from human periodontal ligament, from buccal mucosa. from gingival, and from skin were established from explants. Alkaline phosphatase activity was used as an indicator of the periodontal nature of fibroblasts. Cells were first cultured in DMEM with 0.5% fetal calf serum (PCS) and then incubated for 8 h, and 72 h in fresh DMEM with 10% PCS. Total RNA was isolated and used for Northern blotting with a P 32 ‐labeled KGP cDNA. probe. Total RNA from cultured keratinocytes was used as negative controls. KGF mRNA was found in all cultured fibroblasts. Upon addition of 10% PCS to the cell cultures, an increase in KGF mRNA levels was noticed especially after 72 h. Furthermore. RT‐PCR analysis of material scraped from the tooth root surface indicated the presence of KGF mRNA even in non cultured periodontal ligament cells.