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Changes in TGF‐β 1 levels in gingiva, crevicular fluid and serum associated with periodontal inflammation in humans and dogs
Author(s) -
Skalerič Uroš,
Kramar Barbara,
Petelin Milan,
Pavllia Zlatko,
Wahl Sharon M.
Publication year - 1997
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.1997.tb00192.x
Subject(s) - gingivitis , periodontitis , medicine , beagle , inflammation , gingival and periodontal pocket , transforming growth factor , clinical attachment loss , dentistry , pathogenesis , pathology
Transforming growth factor‐beta (TGF‐β) represents a family of polypeptide growth factors, involved in embryogenesis, inflammation, regulation of immune responses and wound healing. To determine whether TGF‐β contributes to the evolution of periodontal disease, we assayed TGF‐β levels in gingiva and crevicular fluid of patients with gingivitis and periodontitis. In parallel, TGF‐β was quantified m gingival fluid and serum of beagles with experimentally‐induced periodontitis. Disease was monitored by several clinical parameters including Plaque Index, Gingival Index, probing depth, and epithelial attachment loss. Gingival tissues were obtained from 9 patients at the time of periodontal surgery, and gingival fluid samples were collected from an additional population of 10 periodontal patients. In 14 beagles, experimental periodontitis was induced and gingival fluids collected 6 months later. Fluid was collected by paper strips and volume measured by Periotron. Additionally, sera was collected before and 9 months after the ligature‐induced periodontitis in 7 beagles. The levels of TGF‐β 1 were measured by ELISA. In the patients, a significantly higher concentration of TGF‐β 1 was observed both in the gingival tissues and fluid samples obtained from the sites with deeper periodontal pockets than in the less involved sites. In beagles, TGF‐β 1 levels measured in gingival fluid were elevated in moderate disease, declining in fluid samples obtained from the pockets during more advanced experimental periodontitis. Furthermore, with the progression of experimental periodontitis, a decrease in TGF‐β 1 occurred in the sera of the beagle dogs. These data suggest that TGF‐β 1 may play a rǒle in the pathogenesis and diagnosis of periodontal disease, and that its actions can be further explored in an animal model.