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Absence of binding of human salivary glycoprotein to human gingival fibroblast‐like cells in vitro
Author(s) -
Zentner Andrej,
Heaney Thomas G.
Publication year - 1996
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.1996.tb00155.x
Subject(s) - fibroblast , saliva , glycoprotein , in vitro , microbiology and biotechnology , monoclonal antibody , chemistry , antibody , cell culture , biology , immunology , biochemistry , genetics
The aim of this study was to determine whether human high molecular weight salivary glycoprotein binds in vitro to human gingival fibroblastlike cells. Primary monolayer cultures of 2 human gingival fibroblastlike cell lines were incubated with a high molecular weight fraction of salivary glycoprotein which expressed blood group A activity and glycoprotein‐cell binding probed using an FITC‐conjugated mouse monoclonal antibody to human blood group A antigen. Surface fluorescence of protein‐treated cells was found to be no greater than that of untreated or serum‐treated control cultures. As significant binding of salivary glycoprotein to gingival fibroblast‐like cells does not occur in vitro , saliva‐mediated inhibition of fibroblast attachment to hydroxyapatite is not dependent on specific ligand‐lectin interactions.