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Effects of delmopinol on antimicrobial peroxidase systems and lysozyme in vitro and in human whole saliva
Author(s) -
Tenovuo Jorma,
Hannuksela Satu,
LenanderLumikari Marianne
Publication year - 1995
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.1995.tb00168.x
Subject(s) - saliva , lysozyme , chemistry , peroxidase , in vitro , myeloperoxidase , antimicrobial , biochemistry , chromatography , enzyme , immunology , biology , organic chemistry , inflammation
Delmopinol is a new surface‐active agent which can reduce plaque formation and gingivitis. This study was aimed to analyze whether delmopinol (0.0032–0.65 mM) interferes with the activity of two surface‐active oral antimicrobial enzymes, salivary peroxidase and lysozyme. In addition to human whole saliva (pH 5.0 and 6.0), the experiments were done in 0.1 M phosphate buffer (pH 6.0) with purified laetoperoxidase (LPO) and myeloperoxidase (MPO). LPO and MPO were significantly inhibited in buffer by delmopinol concentrations >6.5 mM and 3.2 mM, respectively. No such inhibition was found for total peroxidase activity in mixed saliva. In vitro , delmopinol was found to desorb surface‐bound peroxidases in an active form to the liquid phase. In further analyses, the possible effect of delmopinol on peroxidase‐generated hypoihiocyanite (HOSCN/OSCN − ) was studied in saliva and buffer. No effect was found in buffer, but salivary HOSCN/OSCN declined significantly with 6.5 mM delmopinol. This was obviously due to an enhanced decay of hypothiocyanite. rather than its reduced rate of formation. No deimopinol‐related inhibition of lysozyine occurred in saliva or buffer. The results suggest that high concentration (6.4 mM – 0.2 %) of delmopinol may lower the concentrations of antimicrobial HOSCN/OSCN − in saliva but has no effect on human lysozyme.