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Healing of rat mouth mucosa after irradiation with CO 2 , Nd:YAG, and CO 2 ‐Nd:YAG combination lasers
Author(s) -
Luomanen Marita,
RauhamaaMäkinen Raija,
Meurman Jukka H.,
Kosloff Tuula,
Tiitta Outi
Publication year - 1994
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.1994.tb01184.x
Subject(s) - wound healing , laser , granulation tissue , nd:yag laser , medicine , surgery , irradiation , biomedical engineering , materials science , optics , physics , nuclear physics
The healing process of wounds made by a combination laser was studied in 90 rats. The laser system enabled both separate and combined use of CO 2 , and Nd:YAG laser irradiations. The laser wounds and the control excision wounds made by alligator forceps appeared on both sides of the tongue. Specimens from the wound sites were taken immediately, 6 h, and 1, 2, 4, 7, 11, 21, 28, and 42 days after surgery. The wound‐healing process was studied by macroscopic evaluation before preparing the specimens for light microscopy. Some dilferences were noted in the wound‐healing process among the three groups into which the experimental animals were divided. Tissue coagulation damage was most extensive in the Nd:YAG laser sites, where it was observed in its full extent 4 days after surgery. Epithelial cells were seen to begin to proliferate in all the wounds 6 h after surgery. Re‐epithelialization was completed by between 7 (CO 2 ) and 21 days (Nd:YAG) at all the wound sites. The inflammatory cell infiltration was more prominent in the Nd:YAG and the CO r Nd:YAG combination laser wounds than in the COi and excision wounds during healing. Tissue regeneration occurred faster with less contraction in the combination CO 2 ‐Nd:YAG wounds than in Nd:YAG wounds. The best macroscopic healing result was seen in the CO 2 , wound sites. The combination laser was effective both at cutting and at coagulating tissue. Combining the CO 2 and Nd:YAG laser irradiation into one beam resulted in a greater incision depth than what could have been expected from using the two lasers separately.