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Similarities between Fusobacterium nucleatum and Bacteroides fragilis studied by two DNA probes derived from Fusobacterium nucleatum
Author(s) -
Bolstad Anne Isine,
Kleivdal Hans,
Jensen Harald B.
Publication year - 1994
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.1994.tb01144.x
Subject(s) - fusobacterium nucleatum , fusobacterium , bacteroides fragilis , microbiology and biotechnology , bacteroidaceae , bacteroides , biology , porphyromonas gingivalis , bacteria , genetics , antibiotics
A polymerase chain reaction (PCR)‐amplified oligonucleotide DNA probe corresponding to a Fusobacterium nucleatum Fevl DNA region coding for a 40‐kDa major outer‐membrane protein (OMP) and a randomly cloned 2.1 kb DNA probe were found to recognize DNA from the Gram‐negative bacteria Fusobacterium nucleatum and Bacteroides fragilis on Southern blots and slot blots. The results indicate sequence similarity within the DNA fragments studied. Immunoblots tested with polyclonal antibodies against whole cells of F. nucleatum revealed only weak antigen similarity between these species.