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In vitro antimicrobial susceptibility of different serotypes of Actinobacillus actinomycetemcomitans
Author(s) -
Pajukanta Riitta,
Asikainen Sirkka,
Saarela Maria,
Alaluusua Satu,
JousimiesSomer Hannele
Publication year - 1993
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.1993.tb01124.x
Subject(s) - microbiology and biotechnology , ampicillin , serotype , antimicrobial , ciprofloxacin , agar dilution , cefuroxime , actinobacillus , cephalosporin , penicillin , tetracycline , biology , antibiotics , minimum inhibitory concentration , bacteria , genetics
In vitro susceptibility of Actinobacillus actinomycetemcomitans ( A.a .) serotypes to selected antimicrobial agents was investigated by the agar dilution method on supplemented Mueller‐Hinton test medium. Eighty‐three A.a . strains, 80 recent isolates from 40 periodontally healthy or diseased subjects, and three type strains were included in the study. Serotype a represented 20, serotype b 32, serotype c 17, and serotype e 7 and nontypable 4 of the tested strains. The most effective drugs against all A.a . serotypes in vitro were cefaclor, cefuroxime, tetracycline hydrochloride, doxycycline, trimethoprim‐sulfamethoxazole (cotrimoxazole), and ciprofloxacin, which inhibited 100% of the strains at 4.0 μg/ ml, 4.0 μg/ml, 1.0 μg/ml, 2.0 μg/ml, 0.06 μg/ml, and 0.015 μg/ml, respectively. Serotypes a and e were more susceptible to cafaclor and cefuroxime than were serotypes b and c; 100% of the first two groups were inhibited at 2.0 μg/ml and 1.0 μg/ml. Ampicillin inhibited 92% of the tested strains at 1.0 μg/ml. Serotype b was always susceptible to ampicillin. Metronidazole exhibited the best activity against serotype a strains. The lowest minimal inhibitory concentration values for benzylpenicillin, ampicillin, erythromycin, doxycycline, and metronidazole were encountered among serotype b isolates. The results of the present study indicate minor differences in the in vitro antimicrobial susceptibility patterns of different A.a . serotypes, except to metronidazole. Also, the new oral cephalosporins and cotrimoxazole, rare antimicrobial agents in periodontology, showed promising efficacy against all A.a . strains.