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Epithelial α‐naphthyl acetate esterases in the green vervet monkey gingiva before and after periodontal surgery and during tooth eruption
Author(s) -
Juhl M.,
Holmstrup P.
Publication year - 1993
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.1993.tb01095.x
Subject(s) - esterase , junctional epithelium , epithelium , connective tissue , chemistry , dentistry , frozen section procedure , pathology , enzyme , medicine , biochemistry
To provide enzymatic information on de novo formed junctional (JE) and sulcular epithelium (SE), we performed periodontal surgery on 24 teeth. Ten to 14 days postoperatively, all experimental and 16 control teeth were extracted with adjacent buccal gingiva. In addition, specimens from unerupted and partly erupted teeth containing enamel epithelium (EE) were examined. Fixed cryostat sections were cut in series, stained with HE, or incubated with and without substrate for demonstration of α‐naphthyl acetate esterase activity and for control purposes, respectively. The distribution and intensity of the α‐naphthyl acetate esterase activity of newly reformed JE and SE was identical to that of the original JE and SE, i.e. suprabasal and very strong. In contrast, both the oral gingival epithelium (OGE) and the EE displayed a very weak enzyme reaction. These observations indicate that the presence of α‐naphthyl acetate activity of original and reformed JE and SE is probably site specific and of nondevelopmental origin. Heavy inflammation after healing was associated with enhanced epithelial proliferation of OGE and, in addition, marked esterase activity of these proliferations and corresponding OGE. This points at a possible inflammatory induction of the marked esterase activity seen in JE and SE as well as site‐specific, connective tissue influences. Further investigation is needed to elucidate the effect of inflammation on the esterase activity.