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Influence of phenytoin on cytoplasmic free Ca 2+ level in human gingival fibroblasts
Author(s) -
MODÉER THOMAS,
BRUNIUS GUSTAF,
MENDEZ CARLOS,
JUNTTIBERGGREN LISA,
BERGGREN PEROLOF
Publication year - 1991
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.1991.tb01033.x
Subject(s) - phenytoin , extracellular , fibroblast , cytoplasm , basal (medicine) , chemistry , medicine , endocrinology , pharmacology , biochemistry , in vitro , epilepsy , psychiatry , insulin
— Influence of 5,5‐diphenylhydantoin (phenytoin;PHT) on the cytoplasmic free Ca 2+ concentration, [Ca 2+ ] i , was studied in fura 2 loaded adherent monolayers of human gingival fibroblasts derived from three patients before and after 9 months of PHT therapy. In the patient where gingival overgrowth developed during PHT medication (responder), addition of PHT to gingival fibroblasts derived before PHT medication induced a transient extracellular Ca 2+ dependent increase in [Ca 2+ ] i . In a non‐responder patient, where gingival overgrowth did not develop during the same period of PHT therapy, addition of PHT to gingival fibroblasts derived before the start of medication did not significantly affect [Ca 2+ ] i . Under extracellular Ca 2+ deficient conditions, addition of PHT to serum‐starved fibroblasts derived from the two categories of patients before the medication resulted in an increase in [Ca 2+ ] i . In fibroblasts derived from the responder patient during PHT medication, in contrast to those from the non‐responders (n = 2), the basal level of [Ca 2+ ] i was significantly decreased. The results indicate that, in the cases studied, there is a relationship between PHT induced alterations in [Ca 2+ ] i in gingival fibroblasts and the clinical development of gingival overgrowth.