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High resolution scanning electron microscopy of the subplasmalemmal cytoskeleton in human odontoblasts
Author(s) -
SÖGAARDPEDERSEN BO,
BOYE HANS
Publication year - 1990
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.1990.tb00987.x
Subject(s) - odontoblast , cytoskeleton , chemistry , microbiology and biotechnology , electron microscope , biophysics , materials science , cell , biology , biochemistry , dentin , optics , physics , composite material
Abstract – The subplasmalemmal cytoskeleton in human odontoblasts was studied by high resolution scanning electron microscopy. The odontoblast layer was isolated and exposed to formaldehyde, glutaraldehyde, and OsO 4 for some specimens, while the membraneous structures and soluble proteins in the dental tissue were removed by Zenker's solution and 1% OsO 4 for other specimens, without further fixation of the remaining components. The cytoskeletal elements comprised a dense network of interlacing filaments of different diameters in the cell body. Most cytoskeletal elements were parallel to the axis of the cell processes situated inside the dentinal tubules. The appearance and orientation of the investigated subplasmalemmal cytoskeletal elements was unaffected by the choice of method. Both methods confirm the presence of a subplasmalemmal cytoskeleton in human odontoblasts.