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Localization of lysozyme mRNA in the labial salivary glands by in situ hybridization in Sjögren's syndrome
Author(s) -
KONTTINEN YRJÖ T.,
KULOMAA MARKKU,
SEGERBERGKONTTINEN MARGARETHA,
NORDSTRÖM DAN,
KEINÄNEN RIITTA,
GRÖNBLAD MATS,
MALMSTRÖM MARIA
Publication year - 1990
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.1990.tb00979.x
Subject(s) - lysozyme , in situ hybridization , serous fluid , saliva , messenger rna , immunohistochemistry , microbiology and biotechnology , biology , salivary gland , in situ , ductal cells , pathology , chemistry , medicine , biochemistry , gene , organic chemistry
– In this study, lysozyme mRNA in labial salivary glands has been localized with in situ hybridization technique using 35S‐labeled hen lysozyme cDNA (cDNALZM) as a hybridization probe in normals and in patients with Sjögren's syndrome. 35S‐DNALZM:mRNA hybrids were detected only in acinar serous cells, although lysozyme was identified in ductal cells using immunohistochemical techniques. Our results suggest that the serous acinar cells are the only site of lysozyme synthesis in small salivary glands. The presence of lysozyme in ductal cells may be a result of reabsorption from the saliva or concentration from the blood or surrounding tissues.