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Identification of connective tissue components in healing rat gingiva
Author(s) -
GREVSTAD HANS JACOB
Publication year - 1990
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.1990.tb00936.x
Subject(s) - connective tissue , identification (biology) , medicine , dentistry , pathology , orthodontics , biology , botany
– The aim of the study was to examine connective tissue fiber organization during healing of an incisional wound in rat molar gingiva. Forty rats 50 days old were divided into 10 experimental groups each comprising four animals. Following interdental incisions, healing periods of 11‐20 days, and then 3 H‐proline injection, the animals were sacrificed and specimens prepared for light microscopy, autoradiography, and transmission electron microscopy. Isotope labeling with 3 H‐proline occurred over newly formed collagen in the path of incision, while preexisting tissue was unlabeled. Ultrastructurally, preexisting and new tissue were identified by measurement of collagen fibril thickness. The spatial relationship between new and remaining fibrous tissue in the transitional zone suggests that reunion is established by side‐by‐side alignment of new and severed fibrils.