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Influence of bacterial cell concentration and inorganic anions on lysis of Streptococcus mutans BHT by salivary lysozyme
Author(s) -
TWETMAN SVANTE,
LINDER LARS,
MODÉER THOMAS
Publication year - 1984
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.1984.tb01293.x
Subject(s) - streptococcus mutans , lysozyme , lysis , saliva , microbiology and biotechnology , chemistry , bacterial cell structure , bacteria , biochemistry , biology , genetics
– The aim of this work was to study the influence of bacterial cell concentrations and inorganic anions on lysis of Streptococcus mutans BHT by human salivary lysozyme (HSL). HSL was partly purified from saliva by ion exchange chromatography. The bacteria were grown in a synthetic medium containing 3 H‐thymidine to monitor DNA release. The experiments demonstrated that release 3 H‐thymidine was dependent on the bacterial cell concentration and an apparent K m ‐value corresponding to approximately 2.9 × 10 8 cells/ml was calculated. The influence of I − , Br − , Cl − , F − , HCO 3 − and SCN − on bacteriolysis was studied. All anions tested were slightly inhibitory on the action of HSL. The inhibition varied from 7 to 76% depending on the ion and ionic strength. The order of addition of HSL and sodium chloride caused different lytic responses. This was reflected by the amount of HSL adsorbed by the bacteria.