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Scanning electron and incident light microscopy of giant tubules in red deer dentin
Author(s) -
HALS EINAR,
OLSEN HUGO CATO
Publication year - 1984
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.1984.tb00891.x
Subject(s) - scanning electron microscope , electron microscope , microscopy , dentin , materials science , chemistry , optics , biophysics , anatomy , biology , physics , composite material
– Incisal and cuspal teeth from red deers 2½–6½ yr of age were used in this study. Incident light microscopy was used for screening of the specimens with respect to presence of orifices and exposures of giant tubules. Scanning electron microscopy (SEM) was used for study of structural details. For SEM the specimens were pretreated with a 1% solution of NaCIO for 2 or 20 h. One specimen was in addition exposed to a 35% solution of phosphoric acid for 30 s. Two teeth received no pretreatment. Many incisal/occlusal exposures of giant tubules were more or less blocked by a dense, probably mineralized, substance, whereas others were filled with spherical bodies, probably microorganisms. The walls of the pulpal chambers and root canals revealed an abundance of orifices of giant tubules. They were funnel‐shaped with circular or oblong pulpal outlines, the diameters of which in untreated specimens ranged from 15 to 100 μm.