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Development of aggregating ability in cells of Streptococcus mitis ATCC 903 grown under glucose‐limiting conditions in continuous culture
Author(s) -
ABAAS SALEEM,
HOLME TORD
Publication year - 1982
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.1982.tb01532.x
Subject(s) - lactose , sucrose , chemistry , carbohydrate , limiting , galactose , biochemistry , bacteria , sugar , streptococcus mitis , streptococcus , biology , mechanical engineering , engineering , genetics
– Streptococcus mitis ATCC903 grown under glucose‐limiting conditions in continuous culture did not aggregate upon incubation in 10 m M phosphate buffer at pH 5–7 unless a metabolizable sugar was added. aggregation started 45–50 min after the addition of glucose or sucrose whereas slowly metabolized sugars as gadlactose and lactose required several hours to cause aggregation. Active metabolism of the carbohydrate was a prerequisite for aggregation as indicated by acid formation. Chloramphenicol inhibited the development of aggregating ability in the presence of glucose or sucrose. The addition of a source of nitrogen (peptides and amino acids) enhanced aggregation and shortened the time for development of aggregating ability. No aggregation occurred at 0°C and the ability to aggregate was markedly delayed at 20°C as compared to 30°C. Trypsin treatment of the bacteria abolished aggregation, indicating that surface components of protein or glycoprotein nature contributed to the capacity to aggregate.