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Autoradiographic visualization of glycoproteins and glycosaminoglycans in the epithelio‐mesenchymal interface of developing mouse tooth germ
Author(s) -
HURMERINTA KIRSTI
Publication year - 1982
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.1982.tb00738.x
Subject(s) - basement membrane , extracellular matrix , mesenchymal stem cell , glycosaminoglycan , glycoprotein , microbiology and biotechnology , morphogenesis , chemistry , fucose , epithelium , membrane , biology , biochemistry , genetics , gene
– The turnover of basement membrane macromolecules during tooth morphogenesis and odontobiast differentiation was examined by light microscopic autoradiography using 3 H‐fucose. 35 S‐sulfate and 3 H‐glucosamine. Marked incorporation into the basement membrane was found throughout the progressive development. Pulse‐chase experiments and prelabeling of tissue components indicated that glycoproteins and glycosaminoglycans in the dental basement membrane are mainly derived from the enamel epithelium. During odontobiast differentiation, incorporation was increased at the epithelio‐mesenchymal interface at the site of differentiating mesenchymal cells. From these sites the label also disappeared rapidly. This suggests that the active remodeling of extracellular matrix is related to odontobiast differentiation.