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Enzyme histochemical studies of acid phosphatase isoenzymes in induced heterotopic bone formation in guinea pigs
Author(s) -
NILSEN R.,
MAGNUSSON B. C.
Publication year - 1981
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.1981.tb01712.x
Subject(s) - acid phosphatase , chemistry , tartrate resistant acid phosphatase , enzyme , biochemistry , fluoride , tartrate , incubation , mineralization (soil science) , matrix (chemical analysis) , chromatography , inorganic chemistry , organic chemistry , nitrogen
– A bone induction model was unsed to study the effect of different pretreatments and inhibitors added to the substrate media used for the histochemical demonstration of acid phosphatase activity. Two different acid phosphatases (AcP) were demonstrated. Tje AcP of matrix‐phosphatate activity. Two differet acid phosphatases (AcP) Were demonstrated. The AcP of matrix‐producing cells exhibited resistance to pretreatment with EDTA while it was sensitive to tartrate added to the incubation medium. The AcP in dentin resorbing cells was EDTA sensitive but resistant to tartrate. The AcP activities in both resorbing and matrix‐producing cells were generally sensitive to fluoride, copper and molybdate. In some of the dentinoclasts, Fluoride resistant AcP wsa observed. The results support previous investigations on normal bone formation, suggesting that at least two different isoenzymes of AcP exist. Once of these seemed to be related to matrix production and mineralization of the new heteotopic bone.