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NH 4 C1 and protein metabolism in human gingival fibroblasts
Author(s) -
HELGELAND KRISTEN
Publication year - 1981
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.1981.tb01699.x
Subject(s) - chemistry , intracellular , metabolism , biochemistry , secretion , protein metabolism , ammonia , protein biosynthesis , incubation , inhibitory postsynaptic potential , endocrinology , biology
– The biologic effect of ammonia was studied in cultures of fibroblasts isolated from human gingiva. NH CI in the range 2–20 mu was found to exhibit a concentration dependent growth inhibitory effect, with a delayed action wllich was most pronounced at low concentrations. Concomitant with the growth inhibitor)’effect a significant cellular accumulation of protein was evident. No effect on protein synthesis in general, as measured by G‐protine incorporation, was found, whereas some inhibitory effect on collagen biosynthesis was indicated. Secretion of C‐collagen and other labeled proteins was not affected. The only pronounced effect of ammonia on metabolism of the l4C‐labeled proteins was an inhibition of the intracellular degradation of newly synthesized collagen, the lysosomes being suggested as the site for this degradation.