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Ultrasonic dispersion of pure cultures of plaque bacteria and plaque
Author(s) -
OLSEN INGAR,
SOCRANSKY SIGMUND S.
Publication year - 1981
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.1981.tb01687.x
Subject(s) - eikenella corrodens , microbiology and biotechnology , treponema denticola , sonication , agar plate , bacteroides , bacteria , fusobacterium nucleatum , dental plaque , chemistry , agar , porphyromonas gingivalis , biology , chromatography , genetics
– This study compared th somic sensitivity of 12 Gram‐negative and two Gram‐positive bacteria commonly encountered in plaque associated with periontal diseases. Pure bacterial cultures were groun to standard turbidity, diluted in 1/4 strength jprereduced anaerobically sterilized Ringer's solution, and aliquots dispersed for 0–180s, using and MSE sonic oscillator at 6 μm under 80% N 2 10% H 2 and 10% CO 2 . CO 2 . Viable recoveries were determined on anaerobically cultured trypticase soy 5% blood agar plates. Breakage of T. denticola was assessed by electron microscopy. Gram‐positive organisms tolerated sonication better than Gram‐negative. A. viscosus was more resitant than Strep. sanguis. Gram‐negative bacteria could be divided into groups according to their sensitivity. Eikenella corrodens was most resistant, followed by F. nucleatum. B. asaccharolyticus, Capnocytophaga gingivalis, A. actinomycetemcomitans, a strain (2097) of Group IV Racteroides, and B. melaninogenicus ss. intermedius ressted sonication better than “corroding” Bacteroides and oral Campylobacter. T. denticola, Selenomonas sputigena and Wolinella were most sensitive with viable counts which declined after sonication for 5–10s. Recoveries from plaque taken from five patients with periodontal diseases increased with sonication time, reaching higher values for supragingival than for subgingival samples.