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Method for studying the development, structure and microflora of denture plaque
Author(s) -
BUDTZJÖRGENSEN EJVIND,
THEILADE ELSE,
THEILADE JÖRGEN,
ZANDER HELMUTH A.
Publication year - 1981
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.1981.tb01665.x
Subject(s) - serial dilution , dentures , dentistry , agar , anaerobic exercise , agar plate , dental plaque , medicine , microbiology and biotechnology , biology , bacteria , pathology , physiology , alternative medicine , genetics
– Pieces of sandblasted, self‐adhesive tape were stuck to the fitring surface of maxillary dentures in 17 edentulous subjects with climically normal palatal mucosa. After 1 week, during which the subjects abstained from denture hygience, the tape samples were removed for cultural and microscopical examinations. The microorganisms were suspended in RTF by ultrasonic treatment and total viable anaerobic counts and viable counts of yeasts per cm 2 of tape were calculated after inoculation of serial dilutions on nonselective medium and Sabouraud agar. Total anaerobic viable counts ranged from 4×10 4 to 5×10 8 . By repeated sampling the largest intra‐individual variation was 10‐fold, only. Yeast counts constituted less than 1% of the total viable counts in all but two of the subjects. Light and electron microscopy showed a bacterial plaque o predominantly cocci and rods.