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Sensitivity of different human cell lines in the biologic evaluation of dental resin‐based restorative materials
Author(s) -
HENSTENPETTERSEN ARNE,
HELGELAN KRISTEN
Publication year - 1981
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.1981.tb01283.x
Subject(s) - cell culture , fibroblast , agar , staining , dentistry , amelogenin , human cell , cell , human tooth , microbiology and biotechnology , chemistry , materials science , biomedical engineering , pathology , biology , medicine , biochemistry , enamel paint , genetics , bacteria
— Three different cell lines of human origin, i.c. human epithelica cells (NCTC 2544), and epithelial and fibroblast like cells from human gingiva, were tested for their sensitivity fo three different dental resin‐based restorative materials using four different assay techniques. The techniques applied were cell growth measurements by cell number, 53 Cr‐release, Agar overlay with neutral red staining and colony formation. The ranking of teh cells according to their sensitivity ot the materials varied with the assay technique applied, with no cell line being consistently more sensitive than the others. It is also suggested that the type of medium applied could be important for the result obtained.