Premium
Eugenol: liberation from dental materials and effect on human diploid fibroblast cells
Author(s) -
LINDQVIST LENNART,
OTTESKOG PER
Publication year - 1980
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.1980.tb01266.x
Subject(s) - eugenol , chemistry , fibroblast , nuclear chemistry , chromatography , biochemistry , organic chemistry , in vitro
– The amount of eugenol (4‐allyl‐2‐methoxyphenol) released from four different dental materials immersed in phosphate buffer was measured by gas liquid chromatography. Maximal release of eugenol from zinc oxide‐eugenol cement (ZOE) and IRM® was attained within 5 h and corresponded to 5% and 4%, respectively, of the total amount of eugenol in each material. Both the rates and total amounts of eugenol released from Nobetec® and Opotow® were lower than for ZOE and IRM. Eugenol (0.67 mm) applied to growing cultures of human diploid fibroblasts reduced the number of cells recovered to a value which was 4%. of that found for control cultures which grew to form, monolayers. Confluent monolayers of 3 H‐uridine labeled cell cultures which were incubated for 1 h in the presence of 4 mm eugenol lost approximately 100% of this cytoplasmic label, indicating total cell death.