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Combined extracellular sucrolytic enzyme power from a strain of Streptococcus mutans , and purification results
Author(s) -
AKSNES ASMUND
Publication year - 1978
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.1978.tb00652.x
Subject(s) - levansucrase , dextransucrase , streptococcus mutans , chemistry , strain (injury) , enzyme , extracellular , sucrose , invertase , biochemistry , microbiology and biotechnology , hydrolysis , chromatography , biology , bacteria , bacillus subtilis , anatomy , leuconostoc mesenteroides , lactic acid , genetics
— Streptococcus mutans has been shown to produce extracellular invertase, dextransucrase, and levansucrase. The purpose of this work was to study the relative quantities of these enzymes in pure culture supernatant, and in samples from commonly used purification methods. The strain “Ingbritt” was selected because it is a well‐defined human strain, available, and with well‐known growth requirements. The samples were incubated with sucrose for the determination of free monohexoses, and the polysaccharide from ethanol precipitation was hydrolyzed as previously described. In cell supernatant the inversion effect exerted 75% of total sucrolytic power, the dextransucrase 20% and the levansucrase 596. No method, tested in this work, could separate all the activities completely.