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Demonstration of a K + ‐stimulated and ouabain‐sensitive p ‐nitrophenyl phosphatase activity in enamel‐ and dentin‐forming tissues in the rat
Author(s) -
MÖRNSTAD HÅKAN
Publication year - 1978
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1111/j.1600-0722.1978.tb00602.x
Subject(s) - fluoride , sodium fluoride , chemistry , ouabain , enamel paint , enzyme assay , incubation , phosphatase , enzyme , alkaline phosphatase , phosphate , potassium fluoride , ameloblast , biochemistry , sodium , nuclear chemistry , inorganic chemistry , dentistry , medicine , organic chemistry
— Nitrophenyl phosphate hydrolysis was studied at neutral pH with tissue preparations of the rat secretory and maturation enamel organs and denial pulp. By introduction of inhibitors to nonspecific alkaline phosphatase activity and stimulants to the K + stiinulated and ouabain‐sensitive p ‐nitrophenyl phosphatase activity, the latter‐enzyme activity could be demonstrated. This enzyme activity is generally held to be representative of the enzyme sodium‐ and potassium‐stimulated adenosine triphosphatase. The K + ‐stimulated activity was magnesium dependent and highly sensitive to fluoride. It was inhibited completely by 3 mM fluoride in the incubation medium and about 1 mM produced hall the maximum inhibition. The K + ‐independent enzyme activity was inhibited 50–60% by fluoride in concentrations between 3 and 15 mM. The high fluoride sensitivity of the K + ‐stimulated activity may perhaps help to explain the vulnerability of dental tissues to fluoride.